In vitro studies based on monolayer cell cultures are well established to investigate electrophysiological and genotoxic effects due to external impacts (e.g. chemicals, radiation). They even have the potential to replace animal experiments. Monolayer cultures are easy to handle, however, there is a gap between two-dimensional in vitro models and living organism. Cells within a tissue are organized in a 3D communication network and cell-cell-interactions are not limited to two dimensions.

We have established a scaffold-free three-dimensional cell culture system, which is ready for electrophysiological studies. Dissociated neuronal cells and cardiac myocytes aggregated into spheroids by rotation culturing. Cell-Cell and Cell-ECM interactions established a 3D cellular network that mimics the properties of living tissue in vivo better than conventional monolayer cultures.

Scaffold-free three-dimensional cell culture system: Spheroids

Recent publications:

A. W. Daus, P. G. Layer, C. Thielemann, 2012. A spheroid-based biosensor for the label-free detection of drug-induced field potential alterations. Sensors and Actuators B, 165: 53– 58.

A. W. Daus, M. Goldhammer, P.G. Layer, C. Thielemann, Electromagnetic exposure of scaffold-free three-dimensional cell culture systems, 2011, Bioelectromagnetics, 32: 351–359.

A. Daus, Leben auf dem Mikrochip – Biosensoren auf der Basis von Mikroelektroden-Arrays. Labor&More 8/2012.